Journal: Scientific Reports

Article Title: Global fibroblast activation throughout the left ventricle but localized fibrosis after myocardial infarction

doi: 10.1038/s41598-017-09790-1

Figure Lengend Snippet: Myofibroblasts in situ in the adjacent myocardium. ( A ) Immunofluorescence staining of Fibroblast Activation Protein α, FAP-α, (red), α-actinin (green) and DAPI (blue) in SHAM and MI tissue sections. Scale bars represent 30 µm. ( B ) Western blotting of FAP-α (88-kDa) in tissue from the adjacent and remote myocardium of SHAM and MI. (*p < 0.05: **p < 0.01). (2-way ANOVA with Bonferroni post hoc test).

Article Snippet: Subsequently, the sections were incubated overnight with primary antibodies against FAP-α (orb97039, Biorbyt) and alpha-actinin (A5044, Sigma).

Techniques: In Situ, Immunofluorescence, Staining, Activation Assay, Western Blot